FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326432
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT181000.015.79765194-95
TATACCG32700.015.7553975
CCTAGAC32750.014.8753583
TAGACGG18400.014.5091315
GTCTAGG32550.014.3908351
GTATAGG50550.013.8997531
CTTCTGC216950.013.54688794-95
GTCTATG37850.013.4895531
ACTGTCC28950.013.4282758
TCTGCTT157450.013.40101294-95
GTCTTCT284100.013.08160994-95
GTGTATG51200.012.9913831
TATACTG45000.012.9060565
TGCCGTC503500.012.892858594-95
ACTGAGC53950.012.8487528
GCCGTCT425700.012.83413394-95
CCGTCTT358150.012.63274194-95
CGTCTTC330100.012.55692294-95
GTACCGT31400.012.5293266
CAGTGCC44650.012.2734799
Produced by FastQC (version 0.11.5)