FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14304967
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT521950.014.72435394-95
TGCCGTC635500.014.08689694-95
ACTGTCC40400.014.0266198
TTCTGCT222950.013.94816894-95
CCGTCTT433650.013.8939994-95
GTCTAGG41550.013.7524581
TCTGCTT203100.013.07463994-95
GTATAGG76250.012.776591
CCTAGAC45950.012.6396113
CGTCTTC395800.012.57807294-95
ATGCCGT694300.012.45205694-95
CTAGACA63000.012.4157654
CCTATAC76950.012.2953343
GTCTTCT335700.012.27687794-95
CTGCTTG156100.012.24091394-95
TGCTTGA126300.012.12553794-95
TCTAGAC50650.012.0215783
CTATACT83400.011.906044
GTCTAGA52750.011.8979961
CTTCTGC262550.011.66600994-95
Produced by FastQC (version 0.11.5)