FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18026514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT515000.015.05926194-95
TGCCGTC630750.014.59744594-95
CCGTCTT426650.014.20956894-95
CGTCTTC390350.013.56934894-95
TTCTGCT218450.013.43134894-95
GTCTTCT350650.013.09556994-95
CTTCTGC259950.012.64730294-95
TATACCG60800.012.6320195
ATGCCGT687850.012.43247694-95
TATGCCG767850.012.04587392-93
CTAGACA81800.011.73778254
TCTTCTG315150.011.67291894-95
TATACTG86550.011.5251085
GTCTAGG55850.011.0698961
TAGACGG27250.010.99881655
GTGTATG90750.010.993241
GTCTATG68050.010.7371551
GTATAGG96100.010.7224011
GTACCGT43350.010.5867116
TCTAGAC63750.010.5061253
Produced by FastQC (version 0.11.5)