FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15490716
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG52300.015.3142445
TTCTGCT233550.014.7105894-95
GCCGTCT565100.014.32280394-95
CTTCTGC274200.014.05005594-95
GTCTTCT379250.014.03014994-95
TGCCGTC678700.013.7784794-95
CCGTCTT476650.013.77219294-95
CGTCTTC426600.013.609282594-95
TAGACGG22900.013.49924855
TCTTCTG337000.013.05799494-95
GTACCGT39500.012.9247916
GTCTAGG49200.012.7584621
TCTGCTT206550.012.51766894-95
ATGCCGT743300.011.86576494-95
CTAGACA67450.011.7372454
GTGTATG79250.011.7037361
GTCTATG57450.011.6601641
ACTGAGC76350.011.4104368
GTCCTGG39200.011.3526221
TATGCCG833350.011.25793792-93
Produced by FastQC (version 0.11.5)