FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757435
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG20750.015.80025
GTCTAGG42400.014.5830581
CCTAGAC43900.014.5116443
GTATAGG67050.014.4614591
GCCGTCT382200.013.79254194-95
TATACCG43550.013.6583495
GTGTATG65550.013.5775521
TTCTGCT175050.013.45845994-95
GTCTATG50350.013.3968761
CCGTCTT322950.013.24837894-95
TGCCGTC461950.013.17561194-95
TAGAGCG52200.013.0100855
ACTGTCC34750.012.93898
GTACCGT33550.012.8430376
CGTCTTC301050.012.77303294-95
AGTACCG42450.012.6882815
GTCTTCT261300.012.59204894-95
CTAGACA58400.012.4325754
CTTCTGC205550.012.32733594-95
ATGCCGT502850.012.31352694-95
Produced by FastQC (version 0.11.5)