FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13646155
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT366550.015.95039794-95
TGCCGTC448550.015.10684594-95
CCGTCTT306400.015.05440494-95
TAGACGG21750.014.4255275
GTCTAGG45050.013.723011
CGTCTTC275500.013.46231394-95
TATACCG47050.013.3370975
ATGCCGT492200.012.98219294-95
TATGCCG553500.012.29740692-93
GTCTTCT241000.012.18334794-95
GTCTATG55500.012.0673881
TAGAGCG48550.011.3817455
TATACTG67600.011.3609145
GACTGTG24800.011.3295877
CTAGACA62900.011.1690754
CCTAGAC45900.011.1221923
GTCTTAG41100.011.0535081
ACTGAGC67350.010.8468378
CGTATGC633150.010.79477590-91
TTCTGCT162450.010.73798294-95
Produced by FastQC (version 0.11.5)