FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10186511
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT318800.014.90423294-95
TAGAGCG39600.014.9016435
TGCCGTC384900.014.084743594-95
CCGTCTT266150.014.07794994-95
ACTGTCC28300.014.0667398
GTCTAGG33400.014.023661
GTATAAG49000.013.6693471
CGTCTTC242200.013.65231894-95
GTCTTCT211200.013.58276194-95
TATACTG46300.013.1498615
CCTAGAC37250.013.0771393
GTCTATG39000.012.9708071
TTCTGCT132950.012.9638994-95
CTAGACA48850.012.8483084
GTATAGG55100.012.6660891
TAGACGG16600.012.4137625
ATGCCGT420600.012.35474894-95
CTAAGCG32050.012.2760024
TATACCG35350.012.1886965
CTTCTGC153250.012.14821694-95
Produced by FastQC (version 0.11.5)