FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_POK15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_POK15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19011322
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA170300.015.70441694-95
GTATAGG89550.015.5879391
TGCTTGA214300.015.35410694-95
CCTAGAC62500.015.2146553
GCCGTCT905600.014.63952894-95
CCGTCTT740250.014.25868594-95
TGCCGTC1077500.013.93844594-95
TATACCG55050.013.7829825
TAGAGCG67100.013.7508395
ACTGTCC52550.013.7258498
TCTGCTT342400.013.65886894-95
TTCTGCT386550.013.637670594-95
CTGCTTG266300.013.61351194-95
GTCTAGG60200.013.2278871
CTAAGGT71600.013.2154554
GTCTATG67900.013.0385731
GTGTATG87600.012.8869751
TAGACGG33450.012.8818215
CGTCTTC667350.012.51054694-95
CTAGACA87050.012.3766424
Produced by FastQC (version 0.11.5)