FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_NON60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_NON60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15970404
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG53250.016.3581835
GTCTAGG48700.015.1953961
ACTGTCC40350.014.2757158
GCCGTCT586500.014.27310294-95
TGCCGTC705550.013.99210994-95
TGCTTGA149350.013.54624494-95
GTATAGG85100.013.5390881
CCGTCTT483850.013.43442494-95
GTCCTGG47850.013.1161611
GCTTGAA120550.013.053061594-95
CCTAGAC50650.013.0387033
TTCTGCT263200.012.86449694-95
CCTATAC89000.012.7356213
CTATACT92000.012.7276124
CTGCTTG184050.012.55716894-95
TCTGCTT240500.012.45276594-95
CTAGACA75000.012.1777794
ATGCCGT768350.012.11402394-95
CTAAGGT65450.012.0940514
CGTCTTC446650.011.90584494-95
Produced by FastQC (version 0.11.5)