FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_NON180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_NON180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10055102
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT332850.015.34994294-95
CTAGACA49150.014.9607784
TGCCGTC405100.014.78559794-95
CCTAGAC33200.014.5303873
CCGTCTT270350.014.32549694-95
GTCTAGG33700.013.8975911
TATACTG45950.013.8607315
ACTGTCC25500.013.7739388
TAGACGG15900.013.5485695
GTATAGG53950.013.2821811
TATACCG31150.013.0795835
ATGCCGT440350.012.92669594-95
GCCCTAG32800.012.7082451
TCTAGAC40950.012.5810493
GTGTATG50750.012.5508261
CCTATAC55900.012.4839643
TATGCCG489300.012.28426292-93
CGTCTTC243550.012.22924194-95
GCTTGAA77000.012.163815594-95
TGCTTGA91850.012.16017394-95
Produced by FastQC (version 0.11.5)