FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_NON15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_NON15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20085445
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA215100.018.14798794-95
TGCTTGA281500.017.12818194-95
TCTGCTT494900.017.10504294-95
TTCTGCT586200.016.77786894-95
CTGCTTG360250.016.61460994-95
GTATAGG97900.015.1664581
TATACCG64600.015.0795615
GTCTAGG62000.014.5805271
CTTCTGC677450.014.09623994-95
CCTAGAC61200.013.7763823
CCTATAC103850.013.5760713
CTATACT113000.013.4301434
TAGACGG31450.013.4023115
TAGAGCG63100.013.3598335
ACTGTCC47050.013.3383128
GTACCGT45000.013.2172626
CCGTCTT1217150.013.00961294-95
GCCGTCT1434900.013.00515394-95
GTCTTCT922150.012.90524894-95
CGTCTTC1079050.012.75836994-95
Produced by FastQC (version 0.11.5)