FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965507
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN410420.2938812031672033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT3952.8157956E-972.4941252
ATATGCG5900.069.334582
ACGACTG6100.067.061312
GCCCGTA3104.0369687E-665.9796752
GCCCTAA8100.060.6035542
AGGTCAG6102.1827873E-1160.355182
GTGACCA9600.059.6566282
GTTATCA11000.059.501672
GACTGCG4203.571531E-758.4391442
GCGCGTT4253.8758662E-757.7516252
GACTGAC6554.3655746E-1156.208642
GACGTAC2952.5068584E-455.4676632
AGTTCGT3851.4582647E-553.1264952
CCGCGTA3901.574003E-552.4453852
GTCTGGT8601.2732926E-1147.5667462
GGAACGT7902.728484E-1046.6033672
GATCAGC11450.046.4450842
CGGTTAG5402.0218013E-645.4526672
ATGCGTC4503.668126E-545.4526632
GTTTGCG5452.1542419E-645.0356672
Produced by FastQC (version 0.11.5)