FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_NON60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_NON60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17049830
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285560.1674855409115516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTA23650.027.5682012
CGTGGTA29501.8189894E-1220.7199542
GGTCTAG35700.020.5458372
CGTCTAT28452.910383E-1120.0523532
CGCCTTA28451.2876626E-817.187732
GACCCTA52700.017.0110682
CGCCTAA26601.12037014E-716.851192
CGTTATA36455.638867E-1116.7692362
ACCTCTA63450.016.6978782
CGTACTA42001.8189894E-1216.493742
GGTATTA54700.016.3890932
GCCTCTA60450.016.178362
GGTATAA80700.016.1583122
GACCTTA30553.0986484E-816.006252
GGCTGTA33400.015.8604842
CGTCTAA36100.015.803032
CGCATAA33605.842594E-915.7660762
CGTATTA47150.015.5564452
CGAGTAG42553.6379788E-1115.32286552
CGTTCAG26952.370476E-615.1203142
Produced by FastQC (version 0.11.5)