FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_NON180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_NON180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13837417
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229990.16620876569666146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG32350.023.859032
CGTCCTA34850.020.9818212
CGTATAA33550.020.5840032
CGTTATG24101.708031E-920.227292
GCCCTAA38350.020.1261942
GGTATTA49700.019.6168082
GTCCCTA31957.2759576E-1219.0718962
CGTTCAG23452.7457645E-819.055632
GGTTCTA71000.018.8811762
CGTATAT37100.018.6143742
CGTCTAG22103.0774936E-718.381512
CGTACAG28958.403731E-1018.2418232
CGTGTTA22503.7152313E-718.0547282
CGCTATA22954.5729394E-717.7007142
CGCCTAA23054.7861795E-717.6239222
CGTACAA27851.027729E-817.5036872
GCCCTTA40400.017.0938932
CGGCTTA26751.2370401E-716.7048422
GGTATAG59000.016.5246662
GGGCTAG40400.016.088372
Produced by FastQC (version 0.11.5)