FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_NON15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_NON15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22104567
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN374710.16951700524149602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTA32700.025.0154292
GGTCTAG47100.022.577622
CGCCTTA33900.021.7169342
GTCCCTA40150.019.3550262
GGTATAG97650.019.2668762
CGTATAT63800.019.2320822
CGTGGTA32157.2759576E-1219.0825332
CGTGTAA38900.018.9255542
CGCCATA45100.018.1375712
CGGATAG50150.017.9422722
CGTCTAT37805.456968E-1217.3122652
CCGTATC366350.016.94437894-95
CGTATAG53900.016.6939722
GGTACTA82150.016.4297922
CGTATAA63350.016.140582
GCCTCTA74300.015.9637512
CGTTCTA72200.015.8615842
CGTCTTA60000.015.678422
CGTCCTA50100.015.5110652
CGTCTAG34457.745257E-915.4340482
Produced by FastQC (version 0.11.5)