FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893757
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216700.16806583216978574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT23101.8189894E-1224.64552
CGCTATA21851.8189894E-1124.1943232
CGTCTAG19803.8689905E-922.591712
CGCTCAG24751.0186341E-1021.3594342
CGTTTTA28951.8189894E-1221.0699862
CGTGTAA24351.9172148E-920.0402832
GGTCTAG27300.019.3643232
GTCCCTA25353.1614036E-919.2497392
GGCCTAG25350.019.2497392
GGTATAG62650.018.823422
GGTATTA48100.018.5994132
AGTGTTA26405.2314135E-918.4841252
CGTACTA33301.4551915E-1118.3176022
GGCCTAA38300.018.0497722
CACCGTA20402.8871364E-617.9404742
GCGTCAA21204.1666826E-617.2634752
CGGATAG28601.4088073E-817.062272
GCCCTAG38500.016.8997732
CGTTCAA33703.43789E-1016.8935032
CGTTCAG19654.1976138E-516.5557562
Produced by FastQC (version 0.11.5)