FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18973830
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN320830.169090795058246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT35000.023.3221762
CGTCTAG30350.022.8611152
CGGATAG44650.021.9379942
CGTCTAA24951.07320375E-1021.2657132
CGTATAG45450.020.6538522
CGCTATA31300.018.2553792
CGTTCAG29359.495125E-1018.0776672
GGTGTAT56900.017.2149642
CGCATAG27151.389526E-716.5359822
GGTATTA67450.016.3376262
CGTATTG55050.016.3106962
CGTATAT56700.015.8360452
CGCTATG23401.029166E-515.6976192
CGGGTAG60250.015.5803772
CGTGTAA31554.5163688E-815.5234782
TAGACGG25500.015.5155077
CGAGTAG50350.015.4014382
CGGCCTA37651.5716068E-915.1764512
CGTGTTG35501.1836164E-814.9459032
CCGTATC216600.014.88786494-95
Produced by FastQC (version 0.11.5)