FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18718085
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN316680.16918397368106833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG27500.022.2883722
CGTCTAA22356.202754E-1021.939342
CGTATAG44800.021.8903682
CGTCTAG29901.8189894E-1220.4993422
CGTCTAT35300.019.6785932
CGTCCTA31255.456968E-1219.613772
CGTACTA44650.019.2184182
AGTCTAG44950.018.1810992
CGCTATA31352.246452E-916.9443782
CGTCTTG48900.016.7124822
CCGTATC155250.016.66471394-95
CGTATAA54550.016.4796412
CGGTATG37357.8216544E-1116.410452
GACCTTA35205.966285E-1016.251942
CGTTATA45807.2759576E-1215.1671242
GCCTCTA62100.015.1340812
GGTGCTA78700.015.0571592
GGCCTAG35701.2549208E-814.8797272
CGGATAG44206.366463E-1114.791682
CGCTGTA28604.2685915E-614.2874192
Produced by FastQC (version 0.11.5)