FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941084
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN171760.17277793850248122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA17550.032.4091382
CGTATAA29150.023.6933752
GCCCTTA32250.020.1561152
CGCATAG14557.99482E-519.5457172
CGTTTAG21402.190518E-718.984662
AGCCTTA34750.018.7060362
CGCTATA15353.0395313E-918.5270462
CGCCTAA17551.6020354E-518.5195082
CGGATAG24153.839341E-818.5051292
CGGCGTA15901.57875E-417.8861732
GCCTTTG45700.017.7799432
CGTATAT28156.7484507E-1017.3188632
GGTACTA40350.017.1167742
GCCTCTA34054.0199666E-1016.704272
GCCTCAG24559.243213E-716.5487462
GCCCCAA41102.1827873E-1115.8159292
GGTATAG51750.015.7013212
CGTCTAG15650.002921007815.5759112
CCGTATC85700.015.42028594-95
GACTATA32306.365735E-815.0936852
Produced by FastQC (version 0.11.5)