FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13055608
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN218170.1671082648927572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA24250.025.1415822
CGCTTAA17556.8629197E-720.8438782
CGCTATA19658.875941E-820.6847652
CGTATAG27602.0008883E-1120.6173152
CGTGTAA24101.6952981E-920.2384532
GGTGTAT36350.020.12712
CGTGTTA20901.7007551E-719.4476382
GTCCCTA25303.103196E-919.2785262
CGTCTAA17601.6359454E-518.4752562
CGTTTAG26706.0554157E-918.267672
GGTATAG53500.018.2335222
AGTGTTA24755.061338E-818.0646952
CGTATCG16502.0890427E-417.2435722
CGGATAG28801.5445039E-816.935652
CGCATAG19654.2149684E-516.5478122
GACCCTA41901.8189894E-1216.4910372
GACCTTA24659.603682E-716.489072
CGTGGTA22206.4906126E-616.477932
GGTCCTA44750.016.3490522
GGTCTAC30400.016.0443022
Produced by FastQC (version 0.11.5)