FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11457320
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN189100.16504732345784182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA19050.029.963962
CGGATAG27750.023.5084042
CGTTCTA40900.021.9313582
GGTCTAG26800.021.299012
CGCCTAA19256.909795E-821.1804622
GGTATAA58050.020.3686352
CGTATAA29855.820766E-1119.1227282
CGTTCAG19852.168057E-618.4862212
GCGTCAA22703.921541E-717.9614072
CGCATAG16001.6119637E-417.837922
CGTATTA33653.237801E-1016.9632512
CGGGTAG36305.2750693E-1116.8480932
CGTACTA29251.7991624E-816.7271332
GGTACTA46350.016.71362
GGCCTAG24803.0349838E-816.4404812
AGTGTTA24951.0545664E-616.3416392
CGGTCTA22607.4651543E-616.2367922
CGCCTTA17753.560379E-416.0792522
GGGATAG58900.015.9213062
CGTATAT34157.179551E-915.52096752
Produced by FastQC (version 0.11.5)