FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16468319
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN275770.1674548568071823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA30450.037.38012
GGTCTAG33350.024.3783262
GTCCCTA28650.024.120932
CGTCTAG25355.456968E-1222.4501782
CGTATAG39700.020.4790232
CGTCTAT31005.456968E-1219.669772
CGGGCTA27804.8567017E-1019.0093942
CGTACTA39400.018.5714592
CGTACAA35151.8189894E-1218.5039462
CGTGGTA24704.9403752E-818.103622
CGTGATA27157.4396667E-917.967232
CGTATTA57100.017.7980982
CGTCCTA29901.2823875E-917.6742862
CGCTGTA23104.862468E-717.5977752
AGTGTAC32652.2009772E-1017.430692
CGAGTAG42850.017.0762042
CCGTATC180550.016.9969294-95
GGTGTAT48050.016.9202332
TAGACGG21850.016.8288637
CGGATAG41151.8189894E-1216.7937952
Produced by FastQC (version 0.11.5)