FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n02_AZU15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n02_AZU15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19902500
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN330150.1658836829544027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA32900.024.7267632
GCCTCTA64000.022.2444272
CGTGTTA28001.8189894E-1221.790462
CGTCTAG32600.021.2111642
GGTCTAG39050.020.8325352
GGCCTAG41400.020.6325132
GGTATTA67500.020.4884132
GGTGTAT55250.019.8776342
CGCCTTA33250.019.5731852
CGTTATA45200.018.897922
CGTCTAT32701.0913936E-1118.6584972
CGTGTAC38150.018.1253972
GGTACAT73800.017.6370852
CGGATAG41750.017.5367532
CGTATAA51100.017.5119692
GCCCTAA45150.017.1170522
CGTATCG24207.8651647E-716.808072
CGCTTAG46450.016.6379992
CGTCTAA27001.3560566E-716.571512
TAGACGG27100.016.4888887
Produced by FastQC (version 0.11.5)