FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965507
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGTA8250.013.88682522-23
TCGACAG12050.013.15328724-25
GCCCTAG7700.012.9129131
CTAGTCA10150.012.49238324-25
CAGGGTG12200.012.4342059
GCCGTTA12200.012.4342059
CGCTAGT8200.012.18790422-23
CGCCAGG15750.012.0394699
TAAGCGT11700.011.46264424-25
ATCACCG12850.011.4363249
CGTTCTA12900.011.3919979
CTAGACT7201.1386874E-911.1846434
CGTTACG7200.011.17224522-23
GCGACAA17100.011.12260324-25
CGCAAGT8350.011.09318222-23
TAGACTG8551.4551915E-1111.0803235
TGCTAGA13500.011.01425522-23
TTACGCG10200.010.99678124-25
TTACGAT11050.010.8128924-25
CATTCAC14500.010.7888179
Produced by FastQC (version 0.11.5)