FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_NON60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_NON60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17049830
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT306150.016.15408194-95
ACTGTCC46350.015.3374628
TAGACGG29050.015.1734995
GTATAGG95250.015.0290541
TCTGCTT270750.014.87902894-95
GCCGTCT727850.014.1397694-95
TATACCG56750.013.9476015
CTGTCCG33150.013.8728939
GTCTAGG54150.013.8308321
GTACCGT44300.013.8003976
TGCCGTC869700.013.69180394-95
CCTAGAC54750.013.5918873
TAGAGCG60200.013.541945
CCGTCTT609350.013.30783294-95
GTCTTCT473100.013.303694-95
CTTCTGC356000.013.26634894-95
GTGTATG84550.013.0626721
GTATAAG74950.012.6487631
CGTCTTC550800.012.63153794-95
CTGCTTG193350.012.52566494-95
Produced by FastQC (version 0.11.5)