FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_NON180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_NON180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13837417
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG43900.015.114655
GCCGTCT486350.014.3050194-95
TGCCGTC581600.013.67346594-95
CCGTCTT404950.013.38861594-95
ACTGTCC35850.013.2191838
TTCTGCT210300.013.19467494-95
TAGACGG27300.013.1942755
GTGTATG70600.013.1588791
GTATAGG73100.012.6440081
GTCTAGG42800.012.624921
GTCTTCT313050.012.43669294-95
CGTCTTC370650.012.39637994-95
TATACTG63500.012.3899325
CCTAGAC45200.012.3735363
ATGCCGT629300.012.20023794-95
ACTGAGC69700.012.1706568
TAGAGCG48200.011.8979715
CACTGAG68800.011.8476087
CTTCTGC239900.011.76420494-95
GTATAAG59550.011.7004641
Produced by FastQC (version 0.11.5)