FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_NON15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_NON15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22104567
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG74150.017.0670595
GTCTAGG69200.015.7547241
GCCGTCT905250.015.232841594-95
CCGTCTT758250.015.05446494-95
GTATAGG125800.015.0342341
GTCTTCT567800.014.7994194-95
TTCTGCT343850.014.7828994-95
CGTCTTC666750.014.65028494-95
CTTCTGC401700.014.59482794-95
TAGAGCG74150.014.1905885
ACTGTCC54550.014.1616278
TGCCGTC1081100.013.98259794-95
TAGACGG38850.013.9082485
ACTGAGC100350.013.8851398
TCTTCTG486500.013.51702994-95
CACTGAG103100.013.2389087
GTGTATG110550.013.0347851
CCTAGAC74650.012.95301153
TATACTG106100.012.7317495
GTACCGT53100.012.6739256
Produced by FastQC (version 0.11.5)