FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893757
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG22050.019.559255
GCCGTCT369400.014.9202594-95
TATACCG45600.014.8624565
ACTGTCC32600.014.827578
CCGTCTT309050.014.20724994-95
TGCCGTC442600.014.17117494-95
GTCTAGG42750.013.8593261
CGTCTTC280500.013.69346894-95
GACGGTC27100.013.6397357
GTATAAG65100.013.6153521
TAGAGCG46700.013.59900955
ATAGACG28300.013.5655224
GTCTTCT250550.013.41996294-95
CTTCTGC187400.013.20061794-95
TTCTGCT159300.013.16408894-95
AGTACCG43400.013.1042125
CCTAGAC43500.012.8567843
GTACCGT32350.012.7445836
CTAGACA62100.012.6693534
CTGAGCG26550.012.4991159
Produced by FastQC (version 0.11.5)