FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18973830
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG30900.017.0262375
GCCGTCT574050.016.49170194-95
TGCCGTC710800.015.92264894-95
TATACCG68900.015.6156775
CCGTCTT470050.015.25538894-95
CGTCTTC418250.013.83592294-95
ATGCCGT793700.013.72513394-95
GTCTAGG64450.013.6797971
CCTAGAC67200.012.69615653
TATGCCG898800.012.69204792-93
GTCTATG74450.012.6700411
TTCTGCT249350.012.66832494-95
CTGTCCG29200.012.6640429
ACTGTCC48600.012.5798198
GACTGCG28300.012.3926127
GTCTTCT372300.012.34193294-95
GTACCGT43650.012.1604966
CTAGACA95500.012.1598844
CTGTGCG50550.012.0984349
GTGTATG97900.011.7655771
Produced by FastQC (version 0.11.5)