FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18718085
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC479150.015.37599794-95
GCCGTCT388650.015.25536694-95
CGTCTTC303650.014.26582794-95
CCGTCTT319850.014.24711694-95
ATGCCGT525150.013.695230594-95
TAGACGG29050.013.3786255
GTCTTCT276750.013.10936394-95
TATGCCG604950.012.201892-93
ACTGTCC43450.011.88900858
GACAGTG50350.011.3891527
GTCTAGG61250.011.221321
GTGTATG99100.011.0967651
CTGAGCG42500.011.044259
GGTGTCC71750.011.0307118
TAAGGTG95650.010.9013795
GTCTATG78550.010.7412761
GTATGCC680400.010.71987392-93
CTGTCCG26100.010.7176969
TATACCG71600.010.5251395
TTCTGCT191500.010.4440294-95
Produced by FastQC (version 0.11.5)