FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941084
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT200800.015.25730994-95
TGCCGTC249550.015.13470394-95
CCGTCTT171050.013.43667494-95
ATGCCGT270650.013.12310994-95
TAGACGG15650.013.0213715
GTCTAGG33700.012.7982041
CCTAGAC34700.012.4285673
TATGCCG312050.012.2018692-93
CGTCTTC158350.012.18006194-95
TATACCG38050.011.8323655
GTCTTCT151450.011.2894-95
TAGAGCG36150.011.0121875
GAACCGT33300.010.9571816
CTAGACA49400.010.9367194
TTCTGCT103500.010.85128394-95
GTACCGT24200.010.7696036
GTATGCC348150.010.46705192-93
TAGAGGT46250.010.3494864
GAGTGTC27950.010.3419027
GACGGTC17900.010.3243797
Produced by FastQC (version 0.11.5)