FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13055608
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT465550.015.71115594-95
CCGTCTT380650.015.35610794-95
GTCTAGG42500.015.0551181
TGCCGTC558800.014.74300894-95
CGTCTTC342350.014.16032194-95
TATACCG43050.014.090715
ACTGTCC34850.014.0052948
TTCTGCT182150.013.93150794-95
GTCTTCT291400.013.692739594-95
CCTAGAC42750.013.4141133
TATACTG59750.013.0077465
ATGCCGT619050.012.91771994-95
TAGAGCG47400.012.8975575
CTAGACA58950.012.8631464
CTTCTGC213150.012.78351694-95
TAGACGG22500.012.6376075
GTACCGT31600.012.1464896
GTATAAG57400.012.1379341
GTCTATG50800.011.9422341
ACTGAGC64300.011.8651368
Produced by FastQC (version 0.11.5)