FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11457320
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT309100.014.65035994-95
TGCCGTC377150.014.45736294-95
CCGTCTT255600.013.9899194-95
TAGAGCG41250.012.8683235
CGTCTTC240800.012.77334894-95
GTACCGT28700.012.7146546
ATGCCGT408700.012.61075794-95
TAGACGG20500.012.4844035
TTCTGCT145150.012.42379294-95
TATACCG39900.012.3534535
CTGTGCG31950.012.1674579
GTCTTCT209950.012.06558494-95
TATGCCG457100.011.97511692-93
GACCGTG27000.011.9355067
ACTGAGC57750.011.8170238
GACTGCG19250.011.8169727
GTCTATG49700.011.63507651
AGACCGT31500.011.5844626
GACAGTG36350.011.34254557
GTCTAGG38900.011.3318061
Produced by FastQC (version 0.11.5)