FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16468319
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT457350.016.81727894-95
TAGACGG28750.015.8243485
CCGTCTT375050.015.78821394-95
TGCCGTC559600.015.74723794-95
TATACCG61650.015.2203485
CGTCTTC332250.014.3988394-95
GTCTTCT288400.014.03293594-95
GTCTAGG56300.013.8061131
ACTGAGC77350.013.7231088
CTTCTGC213200.013.05889994-95
CCTAGAC57800.012.8729723
TCTTCTG256050.012.74280594-95
ATGCCGT613150.012.725677594-95
TATGCCG698250.012.69116292-93
GTGTATG86300.012.6863641
GTACCGT42350.012.5320696
CACTGAG84800.012.5173297
CTAGACA78150.012.3710584
TAGAGCG57900.012.2773655
ACTGTCC41600.012.0747278
Produced by FastQC (version 0.11.5)