FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l01_n01_AZU15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l01_n01_AZU15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19902500
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG64900.017.599575
TTCTGCT402600.017.19273294-95
TAGACGG33250.017.104855
GTCTAGG59200.016.0933151
TCTGCTT347400.015.78413194-95
CTTCTGC479900.015.00113594-95
GTCTATG73700.014.7278431
GTACCGT48850.014.6490466
CCGTCTT891800.014.58787494-95
GCCGTCT1063800.014.55257494-95
CGTCTTC778300.014.33125794-95
GTCTTCT657900.014.31020494-95
CTGCTTG248600.014.26946894-95
TAGAGCG65500.014.2545855
CCTAGAC63450.014.1927623
TCTTCTG567800.013.60951194-95
TGCTTGA196250.013.57199994-95
ACTGTCC50750.013.5404888
TGCCGTC1254050.013.37655394-95
CACTGAG90150.013.0897597
Produced by FastQC (version 0.11.5)