FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15697320
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA4500.028.00224928
TCTCGGT4700.027.28237737
CACTCTT8850.026.714782
AGTGTAG5000.025.20146229
GTAGATC5100.024.70424332
TAGATCT5250.024.42515833
GTGTAGA5350.023.97289530
TGTAGAT5750.022.30343631
TTCCCTA3950.022.2091838
CTCGGTG6200.020.32077638
GATCTCG7100.019.32495935
AGATCTC7250.018.3038234
GTGTAGG7650.017.35400217
TCGGTGG7550.016.98584439
TCCCTAC4650.016.9303119
TAGGGAA7650.016.7657320
CCGATTT3250.016.6277482
TCGTGTA7750.016.5492415
GTAGGGA7950.016.416119
TCAGGTA2351.8189894E-1216.2721947
Produced by FastQC (version 0.11.5)