FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28067528
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28350.011.1212342
GTACTCG56200.09.8157644
GCGTGAC36750.09.6216721
TACTCGA57950.09.3214965
AATTGAC48700.09.1971235
GTAGAAC33400.09.0918611
AGTACTC67450.08.7471293
GGAAAAT118600.08.6404211
GAATTGA75100.08.5147674
CACTCGT62950.08.36158635
AATCGAA54350.08.282445
TCAGGTA42550.08.088927
CTCTATA66850.08.07598245
ACTCGTG66850.08.04233336
TCACTCG67050.07.984210534
GGGAAAT59500.07.7975561
GGGGAAT49600.07.7646641
GCGGAAA79250.07.7015521
CTCGAAT25800.07.68194531
GAATCGA39850.07.6278084
Produced by FastQC (version 0.11.5)