FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25812475
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47200.010.865705511
AATCGAG47100.010.0353375
TAATCGA32300.09.8985754
TCAGGTA57650.09.7161777
CTAATCG25650.09.3936563
AATCGAA46600.08.983815
TAGAACA59700.08.92969312
GGAAAAT133700.08.7931551
GAATCGA40650.08.7515184
AAAATCC90300.08.5285773
CAGGTAG62150.08.469748
GGTAGAA62900.08.40423710
GTACTCG37550.08.2747714
AAATCCT97900.08.0035634
AGATCAG102100.07.696384
AATTGAC45200.07.51920755
AGCATAA103350.07.51349835
GAATTGA67550.07.4997024
AGTACTC47600.07.4746073
AGGTAGA72200.07.44624959
Produced by FastQC (version 0.11.5)