FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35270276
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47250.010.85415111
GTACTCG66300.09.3730124
TCAGGTA62100.09.1285667
CGTGACT33950.08.5568822
CAGGTAG65700.08.3544538
GGAAAAT143700.08.2281821
TACTCGA72750.08.1985115
AAAATCC109400.07.94504073
AGTACTC81800.07.9005023
AATCGAA74250.07.76007035
AAAAATC109700.07.73927552
GGTAGAA67900.07.685995610
GCGTGAC45200.07.67332551
TAGAACA69000.07.62826712
GAATCGA64750.07.37190444
GCCGAAT30900.07.36146551
GGGAAAT78650.07.33065131
GAATTGA94400.07.298494
GAAAAAT184150.07.26467281
AATTGAG149150.07.0924585
Produced by FastQC (version 0.11.5)