FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27151943
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36350.09.28208111
CGTGACT24650.08.9531172
AATCGAA50700.08.6122455
GAATCGA44900.08.3243754
GTACTCG47950.08.1235784
TCAGGTA46450.07.89391377
CTCGAAT24800.07.80980061
GAAAAAT141650.07.79063221
GCGGAAA68000.07.78309151
GGAAAAT110200.07.7046511
CACTCGT56900.07.66924835
GCCTAGA26800.07.64058726
AATTGAG113050.07.6052835
AGCCTAG26350.07.514854425
AAAAATC79400.07.45933962
GAATTGA72350.07.43788154
AAAATCC77400.07.4188053
GCGTGAC33300.07.37183241
ATGTCTC115350.07.3142543
GGGAAAT60700.07.2721121
Produced by FastQC (version 0.11.5)