FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26704979
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39900.010.14780311
CGTGACT23300.09.5685762
GGAAAAT127800.08.9697641
TCAGGTA51300.08.8578687
GTACTCG49300.08.7688164
AATCGAA49450.08.2383345
GAATTGA69300.07.96011734
TACTCGA54950.07.946235
GCGTGAC32400.07.71564151
TAGAACA54850.07.66891912
CAGGTAG56900.07.63027578
GAATCGA41700.07.61324264
AAAATCC92000.07.6121053
AATTGAC47550.07.57349875
CTCGAAT24100.07.5694111
GGTAGAA55100.07.51197510
CGGGTAT12750.07.41879221
CACTCGT37100.07.275810735
GAAAAAT157350.07.12781951
AATTGAG114850.07.1138175
Produced by FastQC (version 0.11.5)