FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29053206
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC51550.010.69064711
TCAGGTA60450.09.71274957
CAGGTAG64000.08.927958
TAGAACA65200.08.62493912
AATCGAG48350.08.5653375
GGTAGAA67200.08.368690510
TAATCGA32300.08.2951574
GGAAAAT148700.08.25431
AGGTAGA69950.08.0718169
CTAATCG26100.07.937323
GAATCGA46850.07.64130934
AAAATCC104250.07.62474353
AGATCAG124150.07.54443654
AATCGAA58550.07.4960215
GAATTGA80650.07.4539614
GGGAAAT72850.07.4504451
ATCAGGT121100.07.30059676
CTCGAAT27350.07.24636271
AATTGAC51300.07.1953115
ATCTAAC22000.07.05563936
Produced by FastQC (version 0.11.5)