FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28077251
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27050.010.5730332
GTACTCG56250.09.2866144
GCGTGAC34700.09.0863861
AATCGAA53750.08.8772775
TACTCGA56750.08.8442575
GGAAAAT110650.08.4670961
AGTACTC66950.08.3413073
TAGGGGG19800.08.29530339
GAATTGA77700.08.2587784
GTAGAAC31250.08.13376411
CTCTATA70350.08.02601245
CACTCGT67200.07.96671735
GAATCGA39350.07.9535684
ATTGACC50450.07.93716346
GGGGGGC245150.07.928945543
AATTGAC53250.07.90395365
ACTCGTG71500.07.61360436
GCGGAAA78400.07.61241441
TCACTCG70800.07.434238434
TATCCAG65400.07.3991725
Produced by FastQC (version 0.11.5)