FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31596235
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36750.09.60965511
CGTGACT28450.09.5777422
AATCGAA58400.08.7870125
GGAAAAT137550.08.7268521
GAATCGA47350.08.6068934
GTACTCG61200.08.5354094
AATTGAC50400.08.1275595
TACTCGA63550.08.1103425
GAATTGA79700.08.1079564
GGGAAAT64200.07.89293431
TCAGGTA47700.07.8286747
AAATCGA60750.07.7461844
AAAATCC96600.07.73905563
CACTCGT67250.07.7266935
GCGTGAC40700.07.6361721
CTCTATA69200.07.606777745
GCGGAAA82050.07.60312651
AATCGTA16050.07.57247735
AGTACTC72750.07.52142763
GAAAAAT152800.07.42844961
Produced by FastQC (version 0.11.5)