FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n02_S2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n02_S2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26203612
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32800.011.8089252
GCGTGAC39600.010.8623911
GTACTCG61400.09.7542234
AGTACTC71450.08.8242273
TACTCGA68450.08.8121595
GGAAAAT110300.08.5346961
AATCGAA47750.08.4372445
AATTGAC45800.08.3050495
CTCGAAT23650.08.2846731
GTGACTC48500.08.2641693
CACTCGT65550.08.20162535
ATGTCTC114400.08.18114643
GAATTGA68950.08.0983554
TATCCAG69250.08.0928335
GAATCGA37750.08.0518614
CTCTATA64850.08.04791545
ACTCGTG67750.08.03518236
TCACTCG67100.08.01187834
GATGTCT119450.07.96711442
ATATCCA78650.07.92976764
Produced by FastQC (version 0.11.5)