FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15697320
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA8500.035.17365616
ACGTCTG8600.035.0225915
CACGTCT9500.031.70466214
CACACGT9650.031.67779212
ACACGTC10250.029.82338513
CAGTCAC8150.027.3872427
AGCACAC11550.026.4669810
GCACACG11700.026.12965611
CCAGTCA9400.024.9445826
GAGACCG5400.022.94796431
CCGAGAC5700.022.54490929
CTCCAGT12100.022.35737424
TCGGAAG17500.021.971263
ACCGATC5200.021.61591334
AACTCCA13800.021.38376622
ATCGGAA18100.021.2430672
GATCGGA18450.020.477141
CGAGACC6550.019.61447130
CGGAAGA19500.019.4864964
CTGAACT16650.018.36161419
Produced by FastQC (version 0.11.5)