FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28067528
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA304290.10841353752279147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC42600.025.93378338
ACGTCTG47750.024.64260915
CCGTACG47250.024.38076833
CGTCTGA49400.023.77398116
TCGTATG45750.022.6227445
ACCGTAC54450.021.77576432
CACCGTA53900.021.74809631
CTCGTAT48800.020.88607644
TCGGAAG60800.019.3942383
CACGTCT63900.018.94260814
ATCGGAA63400.018.5279352
CACACGT72400.016.78089312
GTAATCT67650.016.73013939
TACGTAA70100.016.46619636
ACGTAAT70800.016.27161437
CAGTCAC76750.015.74274827
AATCTCG72000.015.4378841
ACACGTC83400.014.83730413
TAATCTC78500.014.64685540
AGTCACC87900.014.18070528
Produced by FastQC (version 0.11.5)