FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25812475
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG41650.030.30599633
GTTTCGG45500.027.8403834
AGTCACG48800.026.23472228
ACGTCTG51000.025.80726215
CGTCTGA53750.024.23579216
TTTCGGA53600.023.75908935
ACGTTTC54650.023.1786432
TTCGGAA55650.022.7229836
TCGTATG43950.022.26901245
CTCGTAT44350.022.16962844
GTCACGT58350.022.13375329
ATCGGAA65750.019.5084922
TCGGAAT65950.019.03765937
TCGGAAG69150.018.9071483
CGGAATC67450.018.61428638
CACACGT76550.017.22309712
CACGTTT76300.017.103431
GGAATCT72450.017.05030839
CACGTCT79850.016.7366214
ACACGTC82400.016.16407213
Produced by FastQC (version 0.11.5)