FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35270276
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA363160.10296488748769644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63100.025.8504415
AGTCACG64100.024.81634728
CGTCTGA68100.024.0185716
TCGTATG60500.023.24334745
GGCCTTA72350.021.89404536
CACGTGG73150.021.46913131
CTCGTAT67050.021.27475244
GTCACGT78450.020.21960329
GCCTTAT82450.019.34850737
ATCGGAA84700.019.2622132
CACGTCT89200.018.86670314
TCGGAAG88000.018.6421183
TATCTCG83050.017.90804541
ACGTGGC90050.017.76435532
TCACGTG94750.016.81233630
CACACGT99650.016.77538912
CAGTCAC100650.016.16247227
CCTTATC99800.015.96226838
CTTATCT99100.015.82539939
ACACGTC111800.015.35472313
Produced by FastQC (version 0.11.5)