FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l08_n01_S2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l08_n01_S2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27151943
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA350350.12903312297024194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45750.023.11335215
AGTCACG44850.022.92604428
TCGTATG46000.021.76586545
CGTCTGA51150.020.54131516
CTCGTAT46800.020.33609244
CGTGAAA51250.020.0198133
CACGTGA53600.019.26733831
ACGTGAA53750.019.21310832
AACGATC50000.018.40574838
GTCACGT56700.018.25366829
TGAAACG59150.017.2318435
TCGGAAG62200.017.1851043
CACGTCT64350.016.95701414
GAAACGA59450.016.8425736
ATCGGAA64800.016.217672
AAACGAT61550.015.75612437
TCACGTG74750.014.08672530
CAGTCAC75250.014.08311227
CACACGT78300.013.82107712
ACACGTC81150.013.668303513
Produced by FastQC (version 0.11.5)